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KMID : 1041219990410030180
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Journal of Sericultural and Entomological Science
1999 Volume.41 No. 3 p.180 ~ p.184
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Chemical Properties of the Insecticidal Compound Produced by an Actinomycetes, SS-4993
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Abstract
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The insecticidal compound produced by Steptomyces SS-4993 strain was indicated to be a relatively stable small compound by pancreatin treatment. The active compound was adsorbed on HP-20 and eluted with 70% acetone. Then, the eluate was extracted with ethyl acetate. The content of the extract was determined by thin layer chromatography (Silica gel 60 F254, hexane-ethyl ether-acetic acid,80 : 20 : 1). Three active bands (Rr 0.08,0.77,0.88) were identified by dermal application using Bombyx mori larvae. Electrophoretic analysis revealed that the injection of strain SS-4993 culture broth into B. mori larvae induced the reduction of the hemolymph proteins level. Especially, the level of apolipophorin-I was decreased drastically.
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